Nobel-prizewinning biologist pioneered use of C. elegans as an animal model.
The Organizing Committee invites you to attend the 18th International C. elegans Meeting, sponsored by the Genetics Society of America. The meeting will be held June 22 – 26, 2011 at the University of California, Los Angeles campus. The meeting will begin on Wednesday evening, June 22 at 7:00 pm and will end on Sunday, June 26 at 12:00 noon. On Friday, June 24 at 5:00 pm there will be a Keynote Address by Joseph Culotti, Samuel Lunenfeld Research Institute, Toronto, Canada
Microscopy, phenotyping and visual screens are frequently applied to model organisms in combination with genetics. Although widely used, these techniques for multicellular organisms have mostly remained manual and low-throughput. Here we report the complete automation of sample handling, high-resolution microscopy, phenotyping and sorting of Caenorhabditis elegans. The engineered microfluidic system, coupled with customized software, has enabled high-throughput, high-resolution microscopy and sorting with no human intervention and may be combined with any microscopy setup. The microchip is capable of robust local temperature control, self-regulated sample-loading and automatic sample-positioning, while the integrated software performs imaging and classification of worms based on morphological and intensity features. We demonstrate the ability to perform sensitive and quantitative screens based on cellular and subcellular phenotypes with over 95% accuracy per round and a rate of several hundred worms per hour. Screening time can be reduced by orders of magnitude; moreover, screening is completely automated.